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Products: Almonds, Peanuts

High-throughput Analysis of Aflatoxins in Cereals, Nuts and Processed Products Involving Automated Immunoaffinity Cleanup and Inline HPLC-Fluorescence Detection.

Authors: Dhanshetty, M., Thorat, P., & Banerjee, K.
  • Journals: J Aoac Int
  • Pages:
  • Year: 2021
Background: The testing of aflatoxins (AFs) in fresh and processed foods is highly demanded to comply with trade regulations. Consequently, commercial laboratories face huge AF sample loads in food consignments. Worldwide, there is a rising interest to implement automation to increase sample throughput in AF analysis. Objective: This study sought to evaluate the performance of an automated cleanup and HPLC analysis system for determination of regulated AFs (B1, B2, G1, G2) in rice, flattened rice, sorghum, raw and processed peanut, almond, peanut butter, and wheat-based cookies. Methods: The samples were extracted with methanol-water (80:20), diluted with Triton X-100 and subjected to automated analysis, where the cleanup step through immunoaffinity column (IAC) and HPLC-fluorescence analyses [involving post-column bromination-derivatisation] were performed in 10 and 11 min, respectively. The method was validated in all test matrices at the LOQ and higher levels. The method performance was also evaluated against a conventional workflow where cleanup and HPLC analysis were manually performed. Results: The LOQ for peanut, sorghum, rice, and flattened rice was 0.125 ng/g, while it was 0.5 ng/g for peanut butter, almond, and wheat-based cookies. In all matrices, the recoveries at LOQ and higher levels were satisfactory. The double-cartridge exchange system completed the analysis of ∼96 injections in 18 h. Each IAC could be reused for 15-times, without incurring any recovery loss. The automated-system provided a better precision (RSD<9%) than the conventional (RSD=12-15%) workflow. Conclusions: Because of its high-throughput nature, this method is recommended for routine analysis of AFs. Highlights: A high-throughput method is reported where cleanup and HPLC analysis of aflatoxins were automatically performed. Each immunoaffinity column could be used 15-times without any loss in recoveries. The method performance was better than the conventional approach and complied with the analytical quality control guidelines. https://doi.org/10.1093/jaoacint/qsab083