Efficient and sustainable recovery of bioactive compounds from cashew nut testa shell (Anacardium occidentale L.) using pressurized liquid extraction
Cashew nut processing generates large quantities of byproducts daily, such as cashew nut testa shell (CNTS), which is discarded, leading to environmental and economic issues. However, CNTS contains bioactive substances such as polyphenols and antioxidant components, with potential for applications in the food, pharmaceutical, and chemical industries. This study investigated the optimization of pressurized liquid […]
In house validation of a high resolution mass spectrometry Orbitrap-based method for multiple allergen detection in a processed model food.
In recent years, mass spectrometry (MS) has been establishing its role in the development of analytical methods for multiple allergen detection, but most analyses are being carried out on low-resolution mass spectrometers such as triple quadrupole or ion traps. In this investigation, performance provided by a high resolution (HR) hybrid quadrupole-Orbitrap™ MS platform for the […]
Cross-reactivity profiles of legumes and tree nuts using the xMAP® multiplex food allergen detection assay.
The homology between proteins in legumes and tree nuts makes it common for individuals with food allergies to be allergic to multiple legumes and tree nuts. This propensity for allergenic and antigenic cross-reactivity means that commonly employed commercial immunodiagnostic assays (e.g., dipsticks) for the detection of food allergens may not always accurately detect, identify, and […]
Multiplex detection of food allergens and gluten. Anal Bioanal Chem.
Detection of hazelnuts and almonds using commercial ELISA test kits
Three commercial sandwich enzyme-linked immunosorbent assay (ELISA) test kits for the detection of hazelnuts and almonds were evaluated. Limits of detection and dynamic ranges were determined for hazelnuts and almonds spiked into cooked oatmeal; dipping chocolate; and muffins (baked). The limit of detection values varied from 1 to 38 mug/g; depending on the food matrix […]
Combined use of total metal content and size fractionation of metal biomolecules to determine the provenance of pine nuts (Pinus pinea)
Four essential elements (Mn; Ni; Zn; and Cu) and their molecular-size distribution patterns have been determined; for twenty four samples of pine nuts from eight areas in Spain and Portugal (Huelva; Cádiz; Badajoz; Cataluña; Castilla; Madrid; Faro; and Coimbra); by size-exclusion liquid chromatography (SEC) coupled on-line to UV and inductively coupled plasma mass spectrometric (ICP-MS) […]