Fast impedimetric immunosensing of IgGs associated with peanut and hazelnut allergens

Food allergies trigger a variety of clinical adverse symptoms and clinical evidence suggests that the presence of food allergy-related IgG can be helpful in the diagnosis when analyzed at the peptide-epitope level. To validate and select the peptides based on their specificity toward hazelnut or peanut epitopes, the authors of this study developed a silicon-based […]

Fast impedimetric immunosensing of IgGs associated with peanut and hazelnut allergens

Food allergies trigger a variety of clinical adverse symptoms and clinical evidence suggests that the presence of food allergy-related IgG can be helpful in the diagnosis when analyzed at the peptide-epitope level. To validate and select the peptides based on their specificity toward hazelnut or peanut epitopes, the authors of this study developed a silicon-based […]

AIEgens enabled ultrasensitive point-of-care test for multiple targets of food safety: Aflatoxin B 1 and cyclopiazonic acid as an example

Food safety is currently a significant issue for human life and health. Various fluorescent nanomaterials have been applied in the point-of-care test (POCT) for food safety as labeling materials. However, previous fluorescent nanomaterials can cause aggregation-caused quenching (ACQ), thus reducing the detection sensitivity. Conversely, aggregation-induced emission luminogens (AIEgens) are promising candidates for POCT in the […]

An aptamer-based dipstick assay for the rapid and simple detection of aflatoxin B1

A rapid and simple dipstick assay based on an aptamer has been developed for the determination of aflatoxin B1 (AFB1). The dipstick assay format was based on a competitive reaction of the biotin-modified aptamer specific to AFB1 between target and cy5-modified DNA probes. Streptavidin and anti-cy5 antibody as capture reagents were immobilized at test and […]

Ultrasensitive nanogold probe-based immunochromatographic assay for simultaneous detection of total aflatoxins in peanuts.

Abstract: An ultrasensitive immunochromatographic (IC) assay for simultaneous detection of total aflatoxins (AFB1; AFB2; AFG1; and AFG2) was developed to meet the requirement for rapidly monitoring aflatoxins in agro-products. The assay was based on a competitive format and its sensitivity was improved by using a novel criterion to screen the optimal amount of monoclonal antibody […]