Identification of two pistachio allergens; Pis v 1 and Pis v 2; belonging to the 2S albumin and 11S globulin family
BACKGROUND: IgE-mediated allergic reactions to pistachio appear to be occurring more frequently; however; little is known about its allergenic proteins. OBJECTIVE: We attempted to identify pistachio allergens and to clone the encoding genes. METHODS: Pistachio proteins were extracted and separated by SDS-PAGE. Immunolabelling was performed with sera from 28 pistachio-allergic individuals. Proteins of interest were […]
Food allergy to peanuts in France – evaluation of 142 observations
BACKGROUND: The increase in frequency of peanut allergy and fatal cases have been reported. OBJECTIVES: The objective of this study is to document the severity of food allergy to peanuts by evaluating the reactive dose of peanuts and to search for the role of peanut oil. METHODS: This study is carried out on the basis […]
Influence of plant lipids on immune responses in mice to the major Brazil nut allergen Ber e 1
BACKGROUND: Lipids; particularly bacterial lipopolysaccharide; can impact on immune responses to proteins; with low doses enhancing type 2 responses. OBJECTIVE: We have examined the influence of natural plant lipid extracts on antibody responses provoked in mice by recombinant Ber e 1; the major allergen in Brazil nuts. METHODS: BALB/c strain mice were immunized (by intraperitoneal […]