Transcriptomic and Metabolic Analysis of Fruit Development and Identification of Genes Involved in Raffinose and Hydrolysable Tannin Biosynthesis in Walnuts.

Walnut (Juglans regia L.) is an important fruit tree with high nutrition in its nuts. Here, the development of walnut fruits was monitored, and nine biological samples at five developmental stages were collected and analyzed by transcriptomic and metabolic assays. Many phenolic metabolites accumulated in the peel of mature fruits, while lipids, carbohydrates, and amino acids […]

Unbiased Immunization Strategy Yielding Specific Nanobodies against Macadamia Allergen of Vicilin-like Protein for Immunoassay Development

Macadamia nut contains important food allergens that potentially cause allergic reactions with severe adverse effects in infants and adults. Reliable and accurate detection of macadamia is critical to avoid allergic reactions. However, knowledge on macadamia allergen is scarce and a reliable detection method has not been reported, yet. In this study, an unbiased immunization and […]

Chemical Composition of the Hazelnut Kernel (Corylus avellana L.) and Its Anti-inflammatory, Antimicrobial, and Antioxidant Activities

The chemical composition of hazelnut kernels (Corylus avellana L.) and their COX-2 inhibitory, antimicrobial, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical-scavenging activities were investigated. Six previously undescribed indoleacetic acid glycosides, hazelnutins A-F (1-6), and five known compounds (7-11) were isolated from the hazelnut kernels. The structures of compounds 1-6 were successfully identified by high-resolution-electrospray […]

Food Profiling: Determination of the Geographical Origin of Walnuts by 1H NMR Spectroscopy Using the Polar Extract.

Walnuts, with their health-promoting ingredients, are among the most popular nuts, and practicable methods for determining their geographical origin are needed to tackle food fraud. Authentic walnut samples (235, Juglans Regia L.) from different harvest years (2016-2019) and countries were analyzed by 1H NMR spectroscopy in combination with chemometric methods to determine their geographical origin. Two sample groups […]

Walnut-Enriched Diet Elevated α-Linolenic Acid, Phytoprostanes, and Phytofurans in Rat Liver and Heart Tissues and Modulated Anti-inflammatory Lipid Mediators in the Liver.

α-Linolenic acid (ALA) and its non-enzymatic oxidized products, namely, phytoprostanes and phytofurans, are found in some nuts. The uptake and deposition of these compounds are not well-defined. Walnut has high ALA and a considerable amount of phytoprostanes and phytofurans compared to other common nuts. When fed to rodents, ALA and eicosapentaenoic acid levels increased in […]

Effects of deficit irrigation, rootstock, and roasting on the contents of fatty acids, phytoprostanes, and phytofurans in pistachio kernels.

Pistachio (Pistacia vera L.) is a drought-tolerant specie grown under the semiarid conditions of the Mediterranean basin. For this reason, it is essential to make an exhaustive quantification of yield and quality benefits of the kernels because the regulated deficit irrigation will allow significant water savings with a minimum impact on yield, while improving kernel […]

Detection of Peanut Adulteration in Food Samples by NMR Spectroscopy.

The addition of cheap and also readily available raw materials, such as peanut powder, to visually and chemically similar matrices is a common problem in the food industry. When peanuts are used as an adulterant, there is an additional risk of potential health hazard to consumers as a result of allergy-induced anaphylaxis. In this study, […]

Phytoprostanes and phytofurans–oxidative stress and bioactive compounds–in almonds are affected by deficit irrigation in almond trees.

Almonds have gained consumers' attention due to their health benefits (they are rich in bioactive compounds) and sensory properties. Nevertheless, information about phytoprostanes (PhytoPs) and phytofurans (PhytoFs) (new plant markers of oxidative stress and compounds with biological properties for human health) in almonds under deficit irrigation is scarce or does not exist. These compounds are […]