Association of Clinical Reactivity with Sensitization to Allergen Components in Multifood-Allergic Children.

BACKGROUND: Thirty percent of children with food allergies have multiple simultaneous allergies; however, the features of these multiple allergies are not well characterized serologically or clinically. OBJECTIVE: We comprehensively evaluated 60 multifood-allergic patients by measuring serum IgE to key allergen components, evaluating clinical histories and medication use, performing skin tests, and conducting double-blind, placebo-controlled food […]

Detection of Food Allergens by Taqman Real-Time PCR Methodology.

Real-time PCR (polymerase chain reaction) has shown to be a very effective technology for the detection of food allergens. The protocol described herein consists on a real-time PCR assay targeting the plant ITS (Internal Transcribed Spacer) region, using species-specific primers and hydrolysis probes (Taqman) dual labeled with a reporter fluorophore at the 5' end (6-carboxyfluorescein, […]

Impact of processing conditions on the phytoprostanes profile of three types of nut kernels.

The goal of this work was the identification and quantification of phytoprostanes (PhytoPs) in three types of nuts: "Walnut", "Macadamia", and "Pecan". This study represents a first approach to the relationship between the quantitative and qualitative PhytoP profiles in the "Macadamia" and "Pecan" nuts subjected to fried salt or fried honey processing. The kernels were […]

Association of tree nut and coconut sensitization.

BACKGROUND: Coconut (Cocos nucifera), despite being a drupe, was added to the US Food and Drug Administration list of tree nuts in 2006, causing potential confusion regarding the prevalence of coconut allergy among tree nut allergic patients. OBJECTIVE: To determine whether sensitization to tree nuts is associated with increased odds of coconut sensitization. METHODS: A […]

A retrospect study into the utility of allergen components in walnut allergy.

Walnut (Juglans regia) and Pecan (Carya illinoinensis) are members of the Juglandaceae family. Walnut and pecan appear similar and share high protein homology with the result that most walnut allergic individuals are also allergic to pecan nut. Walnut is a common allergy in tree nut allergic individuals in the United States and the United Kingdom […]

Toxic and essential elements in five tree nuts from Hangzhou market, China.

In this study a total of thirty-five tree nut samples of walnut, pecan, pine seed, hickory nut and torreya were obtained from 5 farm product markets in Hangzhou, China and investigated for essential (Cr, Mn, Fe, Mo, Cu, Zn, Se, and Sr) and toxic (Al, As, Cd and Pb) elements by inductively coupled plasma-mass spectroscopy […]

A Comparison of Flavor Differences between Pecan Cultivars in Raw and Roasted Forms.

The objective of this research was to explore sensory differences among 8 different pecan cultivars ("Pawnee," "Witte," "Kanza," "Major," "Lakota," "Giles," "Maramec," "Chetopa") in raw and roasted forms. The cultivars were collected from 2 growing seasons (2013 and 2014) and evaluated separately. Trained panelists evaluated each cultivar from each season in raw and roasted forms, […]