Integrated small RNA and mRNA expression profiles reveal miRNAs and their target genes in response to Aspergillus flavus growth in peanut seeds.
Background: MicroRNAs are important gene expression regulators in plants immune system. Aspergillus flavus is the most common causal agents of aflatoxin contamination in peanuts, but information on the function of miRNA in peanut-A. flavus interaction is lacking. In this study, the resistant cultivar (GT-C20) and susceptible cultivar (Tifrunner) were used to investigate regulatory roles of miRNAs […]
Diagnosis of Peanut Allergy Using Continuous Likelihood Ratios.
Background: Peanut allergy (PA) is associated with an economic and psychologic burden on patients and families. Its diagnosis includes tests for peanut specific IgE (sIgE), the values of which usually are categorized as positive or negative using a predefined cutoff (usually 0.35kU/L). With the use of Bayes theorem, this categorization can be replaced with a continuous […]
Consensus Report from the Food Allergy Research and Education (FARE) 2019 Oral Immunotherapy for Food Allergy Summit.
Food allergy is a major health problem affecting 5 to 10% of the population in developed nations, including an estimated 32 million Americans. Despite the large number of patients suffering from food allergies, up until the end of January 2020, no treatment for food allergies had been approved by the U.S. Food and Drug Administration […]
Expression and activation of the steroidogenic enzyme CYP11A1 is associated with IL-13 production in T cells from peanut allergic children.
Activation of the steroidogenic enzyme CYP11A1 was shown to be necessary for the development of peanut-induced intestinal anaphylaxis and IL-13 production in allergic mice. We determined if levels of CYP11A1 in peripheral blood T cells from peanut-allergic (PA) children compared to non-allergic controls were increased and if levels correlated to IL-13 production and oral challenge […]
Epitopes with similar physicochemical properties contribute to cross reactivity between peanut and tree nuts.
Many individuals with peanut (PN) allergy have severe reactions to tree nuts (TN) such as walnuts or cashews. Although allergenic proteins in TN and PN have overall low identity, they share discrete sequences similar in physicochemical properties (PCP) to known IgE epitopes. Here, PCP-consensus peptides (cp, 13 aa and 31 aa) were identified from an […]
Preventing food allergy in infancy and childhood: systematic review of randomised controlled trials.
Background: This systematic review of ways to prevent immediate-onset/IgE-mediated food allergy will inform guidelines by the European Academy of Allergy and Immunology (EAACI). Methods: The GRADE approach was used. Eleven databases were searched from 1946 to October 2019 for randomised controlled trials (and large prospective cohort studies in the case of breastfeeding). The studies included heterogeneous interventions, […]
APPEAL‐1: A multiple country European survey assessing the psychosocial impact of peanut allergy.
Background: Peanut allergy (PA) is a common, potentially life-threatening, and typically lifelong condition with a significant burden of illness. However, information is lacking on how persons with PA (PwPA) and their caregivers perceive the psychosocial impact of living with PA. The Allergy to Peanuts imPacting Emotions And Life study 1 (APPEAL-1) survey, conducted across Europe, investigated […]
The Effect of the Food Matrix on the in vitro Bio-Accessibility and IgE Reactivity of Peanut Allergens.
Scope: Factors such as food processing, the food matrix and antacid medication may affect the bio-accessibility of proteins in the gastrointestinal tract and hence their allergenic activity. However, at present they are poorly understood. Methods and results: Roasted peanut flour was incurred into either a chocolate dessert or cookie matrix and bio-accessibility assessed using an in vitro […]
Algal Biodiesel Production with Engineered Biochar as a Heterogeneous Solid Acid Catalyst.
This study evaluates the use of engineered biochar as a heterogeneous solid acid catalyst for transesterification of algal oil derived from a native microalgal consortium. Biochar derived from sugarcane bagasse, coconut shell, corncob and peanut shell were evaluated for catalytic activity following surface modification. Peanut shell pyrolyzed at 400 °C with the sulfonic acid density […]
Peanut shells-derived biochars prepared from different carbonization processes: Comparison of characterization and mechanism of naproxen adsorption in water.
The ubiquitous appearance of nonsteroidal anti-inflammatory drugs (i.e., naproxen) in water bodies has raised enormous concerns among general public. Development of promising materials for eliminating such contaminants from water environment has attracted much attention in the scientific community. In this study, three (direct, post-treated and pre-treated) methods were developed to prepare biochars (800-PSB, 800-800-PSB, and […]