Non-aflatoxigenic Aspergillus flavus as potential biocontrol agents to reduce aflatoxin contamination in peanuts harvested in Northern Argentina.
Biological control is one of the most promising strategies for preventing aflatoxin contamination in peanuts at field stage. A population of 46 native Aspergillus flavus nonaflatoxin producers were analysed based on phenotypic, physiological and genetic characteristics. Thirty-three isolates were characterized as L strain morphotype, 3 isolates as S strain morphotype, and 10 isolates did not […]
Use of Cold Atmospheric Plasma to Detoxify Hazelnuts from Aflatoxins.
Aflatoxins, produced by Aspergillus flavus and A. parasiticus, can contaminate different foodstuffs, such as nuts. Cold atmospheric pressure plasma has the potential to be used for mycotoxin detoxification. In this study, the operating parameters of cold atmospheric pressure plasma were optimized to reduce the presence of aflatoxins on dehulled hazelnuts. First, the effect of different […]
Reduction of Aflatoxins in Apricot Kernels by Electronic and Manual Color Sorting.
The efficacy of color sorting on reducing aflatoxin levels in shelled apricot kernels was assessed. Naturally-contaminated kernels were submitted to an electronic optical sorter or blanched, peeled, and manually sorted to visually identify and sort discolored kernels (dark and spotted) from healthy ones. The samples obtained from the two sorting approaches were ground, homogenized, and […]
Ochratoxin A in dried grapes in Hamadan province, Iran.
The occurrence of ochratoxin A (OTA) in dried grapes was surveyed in this study. Sixty-six samples of dried grapes (40 currants, 16 sultanas and 10 raisins) were collected from dried grapes factories in Hamadan province, Iran, from October 2012 to March 2013. High-performance liquid chromatography (HPLC) was used to determine OTA in these samples. OTA […]
Automated Aflatoxin Analysis Using Inline Reusable Immunoaffinity Column Cleanup and LC-Fluorescence Detection.
A novel reusable immunoaffinity cartridge containing monoclonal antibodies to aflatoxins coupled to a pressure resistant polymer has been developed. The cartridge is used in conjunction with a handling system inline to LC with fluorescence detection to provide fully automated aflatoxin analysis for routine monitoring of a variety of food matrixes. The handling system selects an […]
Aflatoxins and ochratoxin A in export quality raisins collected from different areas of Pakistan.
During 2012-2014, 170 samples of export quality raisins were collected from different vendors in Pakistan. The collected samples were analyzed for the presence of aflatoxins (AFs) and Ochratoxin A (OTA) contamination using HPLC technique. The LOD and LOQ of AFs/OTA were 0.12/0.10 and 0.36/0.30 µg kg-1, respectively. Only 5% of the samples were contaminated with […]
Quantifying aflatoxins in peanuts using fluorescence spectroscopy coupled with multi-way methods: Resurrecting second-order advantage in excitation-emission matrices with rank overlap problem.
A rapid, simple and inexpensive method using fluorescence spectroscopy coupled with multi-way methods for the determination of aflatoxins B1 and B2 in peanuts has been developed. In this method, aflatoxins are extracted with a mixture of water and methanol (90:10), and then monitored by fluorescence spectroscopy producing EEMs. Although the combination of EEMs and multi-way […]
RNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem
The Food and Agriculture Organization of the United Nations estimates that 25% of the food crops in the world are contaminated with aflatoxins. That represents 100 million tons of food being destroyed or diverted to non-human consumption each year. Aflatoxins are powerful carcinogens normally accumulated by the fungi Aspergillus flavus and A. parasiticus in cereals, […]
Decontamination of Aspergillus flavus and Aspergillus parasiticus spores on hazelnuts via atmospheric pressure fluidized bed plasma reactor
In this study, an atmospheric pressure fluidized bed plasma (APFBP) system was designed and its decontamination effect on aflatoxigenic fungi (Aspergillus flavus and Aspergillus parasiticus) on the surface of hazelnuts was investigated. Hazelnuts were artificially contaminated with A. flavus and A. parasiticus and then were treated with dry air plasma for up to 5min in […]
Risk Assessment on Dietary Exposure to Aflatoxin B₁ in Post-Harvest Peanuts in the Yangtze River Ecological Region
Based on the 2983 peanut samples from 122 counties in six provinces of China's Yangtze River ecological region collected between 2009-2014, along with the dietary consumption data in Chinese resident nutrition and health survey reports from 2002 and 2004, dietary aflatoxin exposure and percentiles in the corresponding statistics were calculated by non-parametric probability assessment, Monte […]