A new approach in sample treatment combined with UHPLC-MS/MS for the determination of multiclass mycotoxins in edible nuts and seeds
Monoclonal antibody-quantum dots CdTe conjugate-based fluoroimmunoassay for the determination of aflatoxin B1 in peanuts
A fluoroimmunoassay towards aflatoxin B1 (AFB1) was presented using quantum dots as the fluorescent label. The CdTe QDs were successfully linked to the monoclonal antibody against AFB1. Based on the conjugated complexes, a novel direct competitive fluorescence-linked immunosorbent assay (cFLISA) was developed for AFB1 detection. The 50% inhibition value (IC50) of the cFLISA was 0.149 […]
Effect of ozone on aflatoxins detoxification and nutritional quality of peanuts
Aflatoxins are a group of secondary metabolites produced by Aspergillus flavus and Aspergillus parasiticus with carcinogenicity, teratogenicity, and mutagenicity. Aflatoxins may be found in a wide range of agri-products, especially in grains, oilseeds, corns, and peanuts. In this study, the conditions for detoxifying peanuts by ozonation were optimized. Aflatoxins in peanuts at moisture content of […]
Removal of aflatoxin B1 in edible plant oils by oscillating treatment with alkaline electrolysed water
Alkaline electrolysed water (AlEW) with different pHs was produced by an electrolysed water generator through adjusting the electric current and voltage. The produced AlEW had a pH above 10, and a low oxidation–reduction potential (ORP) of less than −560 mV. In the current study, the effectiveness of AlEW on decontamination of aflatoxin B1 (AFB1) in […]
Mycobiota, aflatoxins and cyclopiazonic acid in stored peanut cultivars
This study evaluated the presence of fungi and mycotoxins [aflatoxins (AFs), cyclopiazonic acid (CPA), and aspergillic acid] in stored samples of peanut of cultivars Runner IAC Caiapó and cultivar Runner IAC 886 during 6 months. A total of 70 pod and 70 kernel samples were directly seeding onto Aspergillus flavus and parasiticus agar for fungi […]
Polyphasic approach to the identification of Aspergillus section Flavi isolated from Brazil nuts
The aim of this study was to use a polyphasic approach to identify Aspergillus section Flavi isolated from Brazil nuts collected in the Amazon forest: investigation of macro- and microscopic morphology, production of extrolites, heat-resistance fungi, and sequencing of DNA regions. The following Aspergillus section Flavi species were identified: Aspergillus flavus (75.5%), Aspergillus nomius (22.3%), […]
Mycobiota and mycotoxins in Brazil nut samples from different states of the Brazilian Amazon region
The objective of this study was to evaluate the presence of fungi and mycotoxins (aflatoxins and cyclopiazonic acid) in Brazil nut samples collected in different states of the Brazilian Amazon region: Acre, Amazonas, Amapá, and Pará. A total of 200 husk samples and 200 almond samples were inoculated onto Aspergillus flavus-parasiticus agar for the detection […]
Modelling Aspergillus flavus growth and aflatoxins production in pistachio nuts
Aflatoxins (AFs) are the main contaminants in pistachio nuts. AFs production in pistachio has been attributed to Aspergillus flavus. The aim of this study was to apply existing models to predict growth and AFs production by an A. flavus isolated from pistachios as a function of moisture content and storage temperature of pistachios in order […]