Comparative study of concatemer efficiency as an isotope-labelled internal standard for allergen quantification.

Mass spectrometry-based methods coupled with stable isotope dilution have become effective and widely used methods for the detection and quantification of food allergens. Current methods target signature peptides resulting from proteolytic digestion of proteins of the allergenic ingredient. The choice of appropriate stable isotope-labelled internal standard is crucial, given the diversity of encountered food matrices […]

Food allergens in skincare products marketed for children.

Background: The application of preparations containing food allergens can cause percutaneous sensitisation and provocation. The prevalence of food allergens in children's cosmetics is unknown. Objectives: To analyse the prevalence of food allergens in skincare products marketed for children and its association with marketing claims and product price. Methods: We reviewed 276 skincare product ingredient labels for the presence […]

Oxidative Stability of Protease Treated Peanut with Reduced Allergenicity.

Oxidative stability and allergenicity are two major concerns of peanuts. This study evaluated the impact of protease treatment of peanuts on its oxidative stability during storage. The raw and dry-roasted peanut kernels were hydrolyzed with Alcalase solution at pH 7.5 for 3 h. The contents of Ara h 1, Ara h 2, and Ara h […]

Food allergen sensitization patterns in a large allergic population in Mexico.

Introduction and objectives: Although food allergy is recognized as a growing worldwide public health problem, there continues to be limited data on prevalence rates in developing and emerging countries. Most prevalence estimates are based on self-reports, with only few studies using objective assessments. The aim was to analyze the frequency of sensitization to food allergens by […]

The influence of cultural attitudes to nut exposure on reported nut allergy: A pilot cross sectional study.

Food allergies in children have become a common management and diagnostic concern and have a significant influence on general health-related quality of life. We investigated the prevalence of reported nut allergy between populations with different cultural attitudes to nuts during pregnancy and infancy. We conducted a survey to investigate the relationship between cultural differences in […]

Prevalence of IgE‐mediated sensitization in patients with suspected food allergic reactions in Jordan.

Background: Despite the recognition of food allergies as a significant cause of morbidity and a growing public health burden worldwide, there are no epidemiological studies addressing food sensitization and allergy in Jordan. Objective: To conduct an epidemiological study that retrospectively analyzes sensitization data from 3463 patients with suspected immunoglobulin E (IgE)-mediated food allergic reactions who performed specific […]

Late introduction of solids into infants’ diets may increase the risk of food allergy development.

Background: This study investigated risk factors associated with food allergy or food intolerance among school children in two Swedish towns. Methods: Questionnaires were used to collect data on self-reported food allergy or intolerance (SRFA) in children aged 7-8 years from Mölndal in southwestern Sweden and Kiruna in northern Sweden. It included questions about specific food allergy or […]

The Utility of Genomic and Transcriptomic Data in the Construction of Proxy Protein Sequence Databases for Unsequenced Tree Nuts.

As the apparent incidence of tree nut allergies rises, the development of MS methods that accurately identify tree nuts in food is critical. However, analyses are limited by few available tree nut protein sequences. We assess the utility of translated genomic and transcriptomic data for library construction with Juglans regia, walnut, as a model. Extracted walnuts […]

Diagnosis of Peanut Allergy Using Continuous Likelihood Ratios.

Background: Peanut allergy (PA) is associated with an economic and psychologic burden on patients and families. Its diagnosis includes tests for peanut specific IgE (sIgE), the values of which usually are categorized as positive or negative using a predefined cutoff (usually 0.35kU/L). With the use of Bayes theorem, this categorization can be replaced with a continuous […]