A cashew specific monoclonal antibody recognizing the small subunit of Ana o 3.
Food allergies represent a substantial medical liability and preventing accidental exposure to food allergens requires constant attention. Allergic reaction to cashew nuts is frequently serious, and the small 2S albumin, Ana o 3, is an immuno-dominant cashew allergen. Ana o 3 is composed of five alpha helices, contains 2 subunits linked by cysteine disulfide bonds, and remains soluble even […]
Oral Immunotherapy for Multiple Foods in a Pediatric Allergy Clinic Setting.
BACKGROUND: The rising incidence of pediatric food allergy results in significant health care burden and family stress. Oral immunotherapy (OIT) has been shown to induce tolerance to peanut, milk and egg. OIT for other foods, particularly multiple foods simultaneously, has not been thoroughly studied. OBJECTIVE: To summarize our experience with OIT for multiple foods in […]
Peanut allergen reduction and functional property improvement by means of enzymatic hydrolysis and transglutaminase crosslinking.
Enzymatic processing could reduce the allergenicity of peanut proteins while may lose the functional properties. Transglutaminase (TGase) is an enzyme for improving the functional properties of proteins/hydrolysates. No studies have been conducted on peanut hydrolysates that are crosslinked with TGase. In this study, allergenicity and functional properties of peanut protein hydrolysate cross-linked by TGase were […]
Quantitative and kinetic analyses of peanut allergens as affected by food processing.
Peanuts contain four major allergens with differences in allergenic potency. Thermal processing can influence the allergenic properties of peanuts. Until now, a kinetic model has not been reported to assess the changes of soluble allergen (extracted from processed peanuts) content as affected by various thermal processing methods. Our objective is to characterize the reaction kinetics […]
Predictive value of peanut skin prick test, specific IgE in peanut-sensitized children in Singapore.
BACKGROUND: The predictive decision points for both peanut skin prick test (SPT) wheal size and serum IgE concentrations, in peanut-sensitized children, have not been evaluated in Singapore. OBJECTIVE: We aim to derive clinically useful predictive decision points to be used for risk stratification of oral food challenge (OFC) in peanut-sensitized patients. METHODS: Patients with a […]
Allergen components in diagnosing childhood hazelnut allergy: systematic literature review and meta‐analysis
BACKGROUND: Hazelnut-specific IgE antibodies (sIgE) in serum support the diagnosis of hazelnut allergy, but extract-based tests have low diagnostic specificity, commonly leading to over-diagnosis. Measuring sensitization to individual allergen components may enhance the diagnosis of hazelnut allergy. We systematically examined data on diagnostic accuracy of sIgE to commercially available hazelnut components to compare their individual […]
Almond (Prunus dulcis) allergen Pru du 8, the first member of a new family of food allergens.
An almond allergen with 2 known short peptide sequences was reported as the almond 2S albumin but was later suspected to be almond vicilin. However, this allergen was designated by WHO/IUIS. This study aimed to determine the true identity of this elusive almond allergen. cDNAs were synthesized from total RNA of the Nonpareil almond. The […]
SPT predictive values for the outcome of cashew challenges in children.
BACKGROUND: Cashew is a common cause of tree nut allergy in children. To date there have been few studies of diagnostic tests for cashew allergy, and positive predictive values (PPVs) for cashew as well as other tree nuts are largely extrapolated from studies of peanut allergy. How relevant these cut-offs are for cashew has not […]
Pistachio and cashew nut allergy in childhood: Predictive factors towards development of a decision tree.
BACKGROUND: Pistachio and cashew nut, which belong to the same botanical family, are tree nuts that induce serious allergic reactions. OBJECTIVE: We aimed to determine the predictive factors for pistachio and cashew nut reactivity during oral food challenge (OFC). METHODS: A total of 112 pistachio and/or cashew nut sensitized children, aged 58.45 (IQR:40.38-88.32) months, were […]
Peanut allergy and oral immunotherapy.
Peanut allergy is the commonest cause of food-induced anaphylaxis in the world, and it can be fatal. There have been many recent improvements to achieve safe methods of peanut desensitisation, one of which is to use a combination of anti-immunoglobulin E and oral immunotherapy. We have treated 27 patients with anti-immunoglobulin E and oral immunotherapy, […]