Identification and Structure of Epitopes on Cashew Allergens Ana o 2 and Ana o 3 Using Phage Display.
Background: Cashew (Anacardium occidentale L.) is a commercially important plant. Cashew nuts are a popular food source that belong to the tree nut family. Tree nuts are one of the eight major food allergens identified by the Food and Drug Administration in the USA. Allergies to cashew nuts cause severe and systemic immune reactions. Tree […]
TreEAT trial: Protocol for a randomized controlled trial investigating the efficacy and safety of early introduction of tree nuts for the prevention of tree nut allergy in infants with peanut allergy.
Introduction: Children with peanut allergy are at increased risk of developing tree nut allergies, which can be severe and for most lifelong. Introduction of peanut in the first year of life can reduce the risk of peanut allergy; however, prevention strategies for tree nut allergies have not been established. We aimed to test the efficacy […]
Natural History of Hazelnut Allergy and Current Approach to Its Diagnosis and Treatment.
Hazelnut allergy is the most prevalent type of nut allergy in Europe, with symptoms that can range from mild, such as hives and itching, to severe, such as anaphylaxis, particularly in patients who are sensitized to highly stable allergens, such as storage proteins. Compared to other types of food allergies, allergies to tree nuts, including […]
Peanut Allergenicity: An Insight into Its Mitigation Using Thermomechanical Processing.
Peanuts are the seeds of a legume crop grown for nuts and oil production. Peanut allergy has gained significant attention as a public health issue due to its increasing prevalence, high rate of sensitization, severity of the corresponding allergic symptoms, cross-reactivity with other food allergens, and lifelong persistence. Given the importance of peanuts in several […]
Open-label study of the efficacy, safety and durability of peanut sublingual immunotherapy in peanut-allergic children.
Background: Studies are limited on the efficacy of peanut sublingual immunotherapy (SLIT). The durability of desensitization after SLIT has not been well described. Objective: To evaluate the efficacy and safety of 4 mg peanut SLIT and persistence of desensitization after SLIT discontinuation. Methods: Challenge proven peanut-allergic 1-11 year old children were treated with open-label 4 mg peanut SLIT […]
Mitigating the allergenicity of peanut allergen Ara h 1 by cold atmospheric pressure argon plasma jet.
Background: Peanut allergy is recognized as a major food allergy that triggers severe and even fatal symptoms. Avoidance of peanuts in the diet is the main option for current safety management. Processing techniques reducing peanut allergenicity are required to develop other options. Cold plasma is currently considered as a novel non-thermal approach to alter protein […]
Defining the window of opportunity and target populations to prevent peanut allergy.
Background: Peanut allergy affects 1% to 2% of European children. Early introduction of peanut into the diet reduces allergy in high-risk infants. Objective: We aimed to determine the optimal target populations and timing of introduction of peanut products to prevent peanut allergy in the general population. Methods: Data from the Enquiring About Tolerance (EAT; n = 1303; […]
Expression, purification, characterization, and patient IgE reactivity of new macadamia nut iso-allergen.
Structural and functional information about food allergens is essential for understanding the allergenicity of food proteins. All allergens belong to a small number of protein families. Various allergens from different families have been successfully produced recombinantly in E. coli for their characterization and applications in allergy diagnosis and treatment. However, recombinant hexameric 11S seed storage […]
Non-thermal processing of cashews: irradiation reduces allergenicity by altering the structure of Ana o 3.
Traditional thermal processing of cashews not only results in nutrient loss and harmful by-products, but also does not significantly reduce allergenicity. Irradiation could be an important non-thermal processing method to reduce cashew allergens’ allergenicity and retain their nutritional properties. This study aimed to evaluate the effects of gamma irradiation processing on the structure and potential […]
Structure and IgE Cross-Reactivity among Cashew, Pistachio, Walnut, and Peanut Vicilin-Buried Peptides.
Peanut and tree-nut allergies are frequently comorbid for reasons not completely understood. Vicilin-buried peptides (VBPs) are an emerging family of food allergens whose conserved structural fold could mediate peanut/tree-nut co-allergy. Peptide microarrays were used to identify immunoglobulin E (IgE) epitopes from the N-terminus of the vicilin allergens Ara h 1, Ana o 1, Jug r […]