Oral food challenge in IgE mediated food allergy in eastern Mediterranean children

Background: The oral food challenge (OFC) in IgE mediated food allergy causes anxiety both in parents and in patients due to its inherent risks. Objective: Documentation of the rate, spectrum, and predictors of positive reactions is instructive. Methods: Children, who underwent OFC between January 1, 2017 and December 31, 2019 were analyzed. Results: A total […]

Multi-dimensional study of the oral microbiome, metabolite, and immunologic environment in peanut allergy

Background: The oral mucosa is the initial interface between food antigens, microbiota and mucosal immunity, yet little is known about oral host-environment dynamics in food allergy. Objective: To determine oral microbial, metabolic, and immunologic profiles associated with peanut allergy. Methods: We recruited 105 subjects (peanut allergic n=56, healthy subjects n=49) for salivary microbiome profiling using […]

Emerging developments in the forefront of peanut oral immunotherapy

Purpose of review: Despite the COVID-19 pandemic, progress continued in the field of peanut oral immunotherapy over the past 12 to 18 months. Of importance, the first oral immunotherapy product for the treatment of peanut allergy was approved by the US Food and Drug Administration in January 2020. Recent findings: Suggested modifications to the practice […]

Treatment with lipid transfer protein sublingual immunotherapy: slowing down new sensitizations

Food allergy (FA) is a potentially life-threatening condition, food allergen immunotherapy, targeting the underlying mechanisms, is a potentially curative strategy in FA. A 46-year-old woman had an episode of facial angioedema and urticaria after mandarin ingestion and other episode of urticaria, abdominal pain, and facial angioedema after eating hazelnuts and almonds 4 years ago and […]

Effect of deglycosylation on immunoreactivity and in vitro pepsin digestibility of major cashew (Anacardium occidentale L.) allergen, Ana o 1

Major cashew allergen, Ana o 1, was purified in its native form from cashew seeds and subjected to enzymatic deglycosylation using PNGase F to assess the potential role of N-glycans in immunoreactivity. Western and dot blotting with pooled human plasma containing anticashew IgE revealed that deglycosylation increased IgE-binding of Ana o 1. Removal of N-glycans […]