Microbead-based simultaneous fluorometric detection of three nut allergens.

A microbead-based method is developed for the detection of three nut allergens that can occur in food. The bead array constitutes of a suspension of spectrally distinct fluorescent polystyrene microbeads and has been applied for the simultaneous detection of three main and serious allergens (hazelnut, peanut and walnut). The method is based on the simultaneous […]

Application of Multiantigen Profiling To Detect Pecan.

A problem often encountered in the detection and identification of undeclared tree nut food allergens is the lack of analytical methods. This problem is accentuated by the current trend, whereby the primary methods used to detect food allergens are antibody-based enzyme-linked immunosorbent assays (ELISAs) and the development of analyte-specific antibodies takes months. The recently developed […]

Assessment of peanut allergen Ara h1 in processed foods using a SWCNTs-based nanobiosensor.

The goals of this research were to develop a rapid single-walled carbon nanotube (SWCNT)-based biosensor and to employ it to commercial food products for Ara h1 detection. The SWCNT-based biosensor was fabricated with SWCNTs immobilized with antibody (pAb) through hybridization of 1-pyrenebutanoic acid succinimidyl ester (1-PBASE) as a linker. The resistance difference (ΔR) was calculated […]

Caregiver and expecting caregiver support for early peanut introduction guidelines.

BACKGROUND: Recent guidelines recommend early peanut introduction (EPI) beginning around 4-6 months in infants with either severe eczema and/or egg allergy, and around 6 months for all other infants. Caregiver preferences for such practices are unkown. METHODS: We explored preferences for EPI and in-office allergy risk assessment (IRA) through a nationally-representative survey of expecting (n=1000) […]